Detailed information on CES microbial respiration apparatus and electrolytic respiration apparatus
C.E.S(Co-ordinated Environmental Services Respirometer Systems)It is the most powerful biodegradable respiratory system on the market There are 20 electrodes that can be used simultaneouslyConduct biological aerobic or anaerobic gas production experimentsNo need for constant supervision, the transition from oxygen consumption to anaerobic can be completed with simple software operations The accuracy of oxygen production can reach 0.02ppm, and the parallelism of the sample is less than 2% Has GLP certificationStandard configuration can be doneOECD301C OECD 301F OECD302C experiment. If an additional dissolved oxygen pH nitrate redox electrode C-CAP absorbs CO2 probe, the OECD209 OECD301D OECD303A OECD301B OECD302B standard test can be performed.Besides being measurableSoil orin liquidCO2Absorption orO2In addition to the amount produced, this system can also be used to measure the oxygen absorption capacity of aquatic/terrestrial animals. Due to the sealed nature of each reaction vessel and bottle, this system is highly suitable for determining volatile compounds. In anaerobic mode, each test tube can detect gas evolution in a closed container, which can be used for closed bottle experiments and sludge suppression experiments. By using specific control techniques, the system can achieve anaerobic cycle operation, and therefore can be used to evaluate the efficiency of the process.
principle
The sensor includes a plastic or stainless steel dual chamber base, as shown in the figure.(c)The chamber is part of the electrolytic cell and contains copper sulfate solution and water. Through electrodes(d)and(e)The charge transfer between them generates oxygen in the room. Through the hole(h)Can connect toSample bottleThe department. another(b)The room is filled with air as a reference pressure. Two chambers are connected to an electric air valve (not shown in the diagram). These valves are used to balance the pressure between the two chambers at the beginning of the test.(j)(k)(l)consist ofC-CAPProbe.
This probe has two functions. Firstly, the top space of the sampling bottle can be clearedCO2The pressure converter can detect that the gas has been cleared; The second is the ability to measure the amount of absorbed gas. probeThe working principle isCO2Through the exhaust trough of the absorption tank(j)And absorbed into an electrolyte containing absorption tank(k),CO2Reacting with electrolyte, the product is attached to the electrode(l)Detected.
Microorganisms breathe oxygen and convert carbon in solution intoCO2.CO2causeC-CAPOr hydroxide absorption, which will cause pressure changes and be detected by pressure sensors. This change informs the control unit to turn on the DC switch, causing a DC current between the anode and cathode, thereby reducingCuSO4Causing copper to deposit on the cathode and anodeSO32-Combining with water,generateO2Due to the anode(d)keep(c)Room,O2exist(c)The chamber generates, causing the top air pressure to rise until the pressure drop through the sensor is0At this point, turn off the current and record the duration of power on.
What is needed to restore stressO2The quantity is directly proportional to the electrolysis current value and the duration of electrification. The sensitivity of the reaction chamber is determined by the top air pressure. If the correct minimum space above the sample is set, it can be measured1ulThe aboveTop space volumebecome differentTransform. Can provide the largest possible solution before the electrolyte is depleted and needs to be replacedO2The quantity depends on the installation of the sensor headCuSO4The size of the solution storage tank.
advantage:
uIndustrial grade embedded single board computer. This computer ensures the operational reliability of the system under long-term operation and protects the data collected by the system.
uSpare battery. The backup battery ensures system startup and data recording functions, supporting at least6An hour long experiment.
uThe result is thatGLPauthentication. The system has a data storage device that copies and prints raw data from the hard drive every hour. After the experiment, physical testing of the product can be conducted by measuring the quality of the electrodes used, which can verify these data.
uEnvironmental control. In order to eliminate data errors caused by fluctuations in temperature and pressure inside the reactor, a constant temperature water bath is used/oil bath(10-180℃), or equipped with air conditioning(10-50℃)And seal the reactor to eliminate the influence of atmospheric pressure changes.
uStandardization. The design of electronic controls, glassware, and sample racks is highly compatible. This means that the system can be economically retrofitted to adapt to different applications.
uEach test tube can be measuredCO2The release amount,O2The generation amount and gas product generation amount.
uEach reactor is equipped withO2Supply pool.
uAlarm function to alert of reactor leakage.
uInstall pressure overload safety valve.
u20Each reactor is independent,approveAt any time during the processSuspend medication and sampling.
uEffective high-resolution and high oxygen demand calibration equipment.Standard configuration provided75mg/hThe oxygen production rate.If an additional metering pump is added,zuiHigh oxygen supply rate>7095mg/h, Resolution<1ul.
utopCan be attachedAir recirculation pump. Used for samples that are difficult or impossible to stir with a magnetic stirrer.
uassembleconsumeOxygen content andOxygen consumption rateReal time graphics and numerical displays are used.
uEach container has an automatic sampling balancing timer.
uThe chassis is made of glass and stainless steel, which is sturdy, durable, and easy to maintain.
uData output has the function of data format files, so data can be imported into spreadsheets or biological process analysis programs.
specifications
resolution
Oxygen uptake or gas release. The adjustable range is 1 to 200ul.
Carbon dioxide emission (C-CAP absorption probe) 2% fsd 0.2mg @ 100mg fsd
sensitivity
The adjustable range is 1 to 200ul.
data record
The time interval can be adjusted from 1 minute to 12 hours. Event records are optional. Record each oxygen supply time or gas extraction event.
Gas supply or extraction
Electrolytic cells are used for concentrations below 75mg/hour, while metering pumps are used for concentrations above 5000ml/hour.
Maximum gas supply: greater than 5000ml/hour
C-CAPprobe2- 3mg CO2The concentration is300ppm>Using gas circulation pump100mg/minute.
Experimental running time
Zui Da: Infinite
Zui Xiao: 5 minutes
Automatic balancing timer
1 minute to 24 hours
accuracy
Electrolytic cell<1%
C-CAP probe+/-2% fsd
Metering pump<2%
System timing<0.0001%
Accumulated pressure of sensors < 0.025%
Built in backup battery time
Zui is 6 hours shorter
Zui is 12 hours long
Pressure vessel size
Zui Xiao: 25ml
The biggest: unlimited
temperature control
Water bath or oil bath-20 to 180C+/-0.1C
air-condition temperature10 arrive50C +/-1C
User defined cooling capacity
User defined heating capacity, usually displayed as 1KW
Power requirements
Control and recording system with 110 Vac 60Hz @ 1.8Amps
240 Vac 50Hz @ 0.8Amps
Water bath 110Vac 60Hz @ 10Amps peak load
240Vac 50Hz @ 5Amps peak load
Air conditioner 110 Vac 60Hz @ 5 Amps
240 Vac 50Hz @ 2.5 Amps
computer
Need a Pentium processor 500 or faster to runWindows 98,ME, 2000, NTOr Xp system.